Rabbit Anti-Human Phospho-SMAD4(T277) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13485 |
| Gene Id | 4089 |
| Gene Symbols | SMAD4 |
| Protein Name / Synonyms | Phospho-SMAD4(T277) |
| Clonality | Polyclonal |
| Preparation | This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF, Dot Blotting, IHC-P |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4
NCBI Accession #
NP_005350.1
Other Accession #
O70437; P97471
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
60439
Recommended Dilutions
IF: 1:10-50; DB: 1:500; IHC: 1:10-50
 | Confocal immunofluorescent analysis of Phospho-SMAD4-T277 Antibody(Cat# 102-15354) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | Dot blot analysis of anti-Phospho-SMAD4-T277 Antibody (RB7971) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
 | Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4-T277 (center) (Cat# 102-15354), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.