3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Phospho-STAT1-S727 Antibody
Catalog #: 144-50109
Phospho-STAT1-S727 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42224 |
| Gene Id | 6772 |
| Gene Symbols | STAT1 |
| Protein Name / Synonyms | STAT1, CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, signal transducer and activator of transcription 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
83kDa/87kDa
Observed MW
87kDa
Immunogen
A synthetic phosphorylated peptide around S727 of human STAT1 (NP_009330.1).
Modification
Phospho S727
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines,using Phospho-STAT1-S727 antibody (144-50109) at 1:2000 dilution. 293T cells and C6 cells were treated by UV at room temperature for 15-30 minutes..
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.