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Anti-Phospho-Stat3-Y705 Antibody
Catalog #: 144-50070
Phospho-Stat3-Y705 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P40763 |
| Gene Id | 6774 |
| Gene Symbols | STAT3 |
| Protein Name / Synonyms | STAT3, ADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
83kDa/87kDa/88kDa
Observed MW
105kDa
Immunogen
A synthetic phosphorylated peptide around Y705 of human Stat3 (NP_644805.1).
Modification
Phospho Y705
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-Stat3-Y705 antibody (144-50070) at 1:2000 dilution or STAT3 antibody (144-01192). HeLa cells were treated by IFN-? (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.