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Anti-Phospho-STMN1-S16 Antibody
Catalog #: 144-50229
Phospho-STMN1-S16 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16949 |
| Gene Id | 3925 |
| Gene Symbols | STMN1 |
| Protein Name / Synonyms | STMN1, C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, stathmin |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
17kDa/19kDa
Observed MW
15kDa
Immunogen
A synthetic phosphorylated peptide around S16 of human STMN1 (NP_001138926.1).
Modification
Phospho S16
Storage
-20°C
Images
Western blot analysis of extracts of HeLa and C2C12 cells, using Phospho-STMN1-S16 antibody (144-50229) at 1:2000 dilution. HeLa cells were treated by Nocodazole (50ng/ml) for 20 hours.C2C12 cells were treated by Nocodazole (50ng/ml) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.