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Anti-Phosphotyrosine Antibody (p-Tyr)
Catalog #: 188-10885
Mouse anti-Phosphotyrosine Antibody (p-Tyr)
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Host Species | Mouse |
| Protein Name / Synonyms | Phosphotyrosine |
| Immunogen | P-Tyr conjugated to BSA was used as the immunogen for the Phosphotyrosine antibody. |
| Isotype | IgG2b |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) |
| Domain / Region / Terminus | Depends upon the location of phosphorylated target |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Storage
Store the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.