Anti-PIAS2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O75928
Gene Id	9063
Gene Symbols	PIAS2
Protein Name / Synonyms	PIAS2, ARIP3, DIP, MIZ, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, E3 SUMO-protein ligase PIAS2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

45kDa/63kDa/68kDa

Observed MW

75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 483-572 of human PIAS2 (NP_775298.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse testis, using PIAS2 antibody (144-05654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - PIAS2 antibody (144-05654)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products