RayBiotech

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ISO 13485:2016 cGMP

Anti-PIEZO1 Antibody

Catalog #: 144-04340

PIEZO1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q92508
Gene Id
9780
Gene Symbols
PIEZO1
Protein Name / Synonyms
PIEZO1, DHS, FAM38A, LMPH3, Mib, piezo-type mechanosensitive ion channel component 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
286kDa
Observed MW
300kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2230-2420 of human PIEZO1 (NP_001136336.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PIEZO1 antibody (144-04340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PIEZO1 antibody (144-04340)

Expiration:
12 months from the date of shipment when stored properly.