Anti-PIGR (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01833
Gene Id	5284
Gene Symbols	PIGR
Protein Name / Synonyms	PIGR (C-term)
Clonality	Polyclonal
Preparation	This PIGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-672 amino acids from the C-terminal region of human PIGR.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Specificity

Other Names

PIGR; Polymeric immunoglobulin receptor; Hepatocellular carcinoma-associated protein TB6; Secretory component

NCBI Accession #

NP_002635.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

83284

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of PIGR Antibody (C-term) (Cat# 102-17915) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	PIGR Antibody (C-term) (Cat# 102-17915)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PIGR Antibody (C-term) (Cat# 102-17915) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Flow cytometric analysis of HepG2 cells using PIGR Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.