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Anti-PIK3C3/hVPS34 Antibody
Catalog #: RB-08-0037
Rabbit Anti-Human PIK3C3/hVPS34 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_002647.2 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human hVPS34. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CGKLFHIDFGYILGR |
| Recommended Applications | ELISA (recommended work dilution= 1:32, 000 (Minimum detecting ability 30 ng/ml)) |
| Reconstitution | Supplied as 200 µg of purified and lyophilized antibody. The antibody is stable for at least one year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggesting with sterile water) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The Class III PI3K (phosphoinositide 3-kinase), Vps34 (vacuolar protein sorting 34), was first identified in yeast, then it was also defined in mammalian cells of different species, including human, designated as hVPS34. Investigations shown that it could play an important role in the ability of cells to respond to changes in nutrient conditions. In both yeast and mammalian cells, Class III PI3Ks are also required for the induction of autophagy during nutrient deprivation. Finally, mammalian hVPS34 is itself regulated by nutrients. Thus Class III PI3Ks are implicated in the regulation of both autophagy and, through the mTOR pathway, protein synthesis, and thus contribute to the integration of cellular responses to changing nutritional status.
Specificity
The rabbit anti-human hVPS34 antibody detects specifically synthetic antigen derived from human hVPS34 at various concentrations. Reactivity with cellular proteins is not tested yet. Cross reactivity with other species are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- Yan Y, Flinn RJ, Wu H, Schnur RS, Backer JM. hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Biochem J. 2009 Feb 1;417(3):747-55. PMID: 18957027
- Gulati P, Gaspers LD, Dann SG, Joaquin M, Nobukuni T, Natt F, Kozma SC, Thomas AP, Thomas G. Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34. Cell Metab. 2008 May;7(5):456-65. PMID: 18460336.
- Backer JM. The regulation and function of Class III PI3Ks: novel roles for Vps34. Biochem J. 2008 Feb 15;410(1):1-17. PMID: 18215151.
Expiration:
12 months from the date of shipment when stored properly.