PIK3CA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42336 |
| Gene Id | 5290 |
| Gene Symbols | PIK3CA |
| Protein Name / Synonyms | PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
124kDa
Observed MW
115-120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PIK3CA antibody (144-00265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody (144-00265) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PIK3CA antibody (144-00265) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PIK3CA antibody (144-00265) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PIK3CA antibody (144-00265) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.