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ISO 13485:2016 cGMP

Anti-PIK3CG Antibody

Catalog #: 144-00266

PIK3CG Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P48736
Gene Id
5294
Gene Symbols
PIK3CG
Protein Name / Synonyms
PIK3CG, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaAngiogenesis, Inflammation
Shipping TypeBlue ice
Storage-20°C

Description

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
126kDa
Observed MW
126kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human PIK3CG (NP_002640.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using PIK3CG antibody (144-00266) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PIK3CG Polyclonal Antibody (144-00266)

Expiration:
12 months from the date of shipment when stored properly.