Anti-PIK3R1 (Y580) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P27986
Gene Id	5295
Gene Symbols	PIK3R1
Protein Name / Synonyms	PIK3R1 (Y580)
Clonality	Polyclonal
Preparation	This PIK3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-587 amino acids from human PIK3R1.
Concentration (lot specific)	0.350 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Xenopus

Other Names

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

NCBI Accession #

NP_001229395.1;NP_852556.2;NP_852664.1;NP_852665.1

Other Accession #

Q63787; P26450; P23727; Q8UUU2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

83598

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of PIK3R1 (arrow) using rabbit polyclonal PIK3R1 Antibody (Y580) (Cat# 102-17763). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products