Anti-PIN1 (Center) Antibody

Product Description

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13526
Gene Id	5300
Gene Symbols	PIN1
Protein Name / Synonyms	PIN1 (Center)
Clonality	Polyclonal
Preparation	This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the Central region of human PIN1.
Concentration (lot specific)	0.423 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

PIN1 is an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis.

Predicted Reactivity

Monkey

Other Names

PIN1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; Rotamase Pin1

NCBI Accession #

NP_006212.1

Other Accession #

Q4R383

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

18243

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of PIN1 Antibody (Center) (Cat# 102-18094) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	PIN1 Antibody (Center) (Cat# 102-18094) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of PIN1 (arrow) using rabbit polyclonal PIN1 Antibody (Center) (Cat# 102-18094). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene.
	Confocal immunofluorescent analysis of PIN1 Antibody (Center) (Cat# 102-18094) with 293 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).