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Anti-PIP4K2B Antibody
Catalog #: 144-08016
PIP4K2B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P78356 |
| Gene Id | 8396 |
| Gene Symbols | PIP4K2B |
| Protein Name / Synonyms | PIP4K2B, PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, PIP5P4KB, phosphatidylinositol 5-phosphate 4-kinase type-2 beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
32kDa/47kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PIP4K2B (NP_003550.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (144-08016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using PIP4K2B antibody (144-08016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PIP4K2B antibody (144-08016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2B antibody (144-08016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PIP4K2B antibody (144-08016) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.