3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-PITRM1 Antibody
Catalog #: 144-08362
PITRM1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q5JRX3 |
| Gene Id | 10531 |
| Gene Symbols | PITRM1 |
| Protein Name / Synonyms | PITRM1, MP1, PreP, pitrilysin metallopeptidase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
106kDa/117kDa
Observed MW
117kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 758-1037 of human PITRM1 (NP_055704.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PITRM1 antibody (144-08362) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using PITRM1 antibody (144-08362) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.