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ISO 13485:2016 cGMP
Anti-PKC gamma Antibody
Catalog #: 131-0145
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1000 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P05129 |
| Gene Id | 5582 |
| Gene Symbols | PRKCG PKCG |
| Protein Name / Synonyms | PKC gamma (305-308aa) |
| Clonality | Polyclonal |
| Immunogen | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen PKC gamma (305-308aa): CNYPLELYERVRMG. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen Sequence | CNYPLELYERVRMG |
| Recommended Applications | ELISA (recommended work dilution = 1:20, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | PKC Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Western Blot
10% SDS-PAGE
- Mouse brain tissue 10 µl
- PKCG: 0.25 µg
Indirect Fluorescent Antibody (IFA) Analysis
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: cell membrane, cytoplasm, perinuclear region
CY3 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: cell membrane, cytoplasm, perinuclear region
Storage/Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.