Anti-PKM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P14618
Gene Id	5315
Gene Symbols	PKM
Protein Name / Synonyms	PKM, CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, pyruvate kinase PKM
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

56kDa/57kDa/58kDa

Observed MW

65kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM (NP_002645.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PKM antibody (144-013905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - PKM antibody (144-013905)

Expiration:

12 months from the date of shipment when stored properly.

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