Anti-PLA2G2D Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q9UNK4
Gene Id	26279
Gene Symbols	PLA2G2D
Protein Name / Synonyms	PLA2G2D, PLA2IID, SPLASH, sPLA2-IID, sPLA2S, phospholipase A2 group IID
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

16kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PLA2G2D antibody (144-07165) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PLA2G2D antibody (144-07165)

Immunofluorescence analysis of U2OS cells using PLA2G2D antibody (144-07165). Blue: DAPI for nuclear staining.
Immunofluorescence - PLA2G2D antibody (144-07165)

Expiration:

12 months from the date of shipment when stored properly.

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