PLA2R1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13018 |
| Gene Id | 22925 |
| Gene Symbols | PLA2R1 |
| Protein Name / Synonyms | PLA2R1, CLEC13C, PLA2-R, PLA2G1R, PLA2IR, PLA2R, phospholipase A2 receptor 1 |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 870-1130 of human PLA2R1 (NP_031392.3). |
| Isotype | IgG |
| Purification | Affinity purification |
| Format | Liquid |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Calculated MW
169 kDa
Observed MW
180 kDa
Dilutions
WB 1:500 - 1:1000
ELISA Recommended starting concentration is 1 μg/mL
ELISA Recommended starting concentration is 1 μg/mL
Storage/Stability
Store at -20℃. Avoid freeze / thaw cycles.
Expiration
12 months from the date of shipment when stored properly.
Background
This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.
Images
Western blot analysis of lysates from A-549 cells, using PLA2R1 antibody (144-10068) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat liver using PLA2R1 antibody (144-10068) at dilution of 1:100 (40x lens).
