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Anti-Platelet-derived growth factor subunit B (PDGFB) Antibody
Catalog #: 130-10139
Rabbit Anti-Platelet-derived growth factor subunit B( PDGFB) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_002599 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 32, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein PDGFbeta. The membrane blot was probed with anti- PDGFbeta primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein PDGFbeta from E.coli cell (12kDa). |
| Antigen Catalog Number | 230-00219 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Platelet-derived growth factor beta (PDGFbeta) is a member of Platelet-derived growth factor family which plays an important role in regulating cell proliferation, cellular differentiation, cell growth, development and many diseases including cancer.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat PDGFbeta has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -83°C. After reconstitution, it can be aliquoted and stored
Related Products
RayBio® Human PDGF-BB ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine (Cat: ELH-PDGFBB-001)
References
- Michael A Beazely, et al. Abelson tyrosine kinase links PDGFbeta receptor activation to cytoskeletal regulation of NMDA receptors in CA1 hippocampal neurons. Mol Brain. 2008; 1: 20.
- Jennifer B. Ptacek, et al. Packing contacts can mediate highly specific interactions between artificial transmembrane proteins and the PDGFbeta receptor. Proc Natl Acad Sci U S A. 2007 July 17; 104(29): 1194511950.
Expiration:
12 months from the date of shipment when stored properly.