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Anti-Platelet-derived growth factor subunit B (PDGFB) Antibody, Biotinylated
Catalog #: 130-10139B-50
Rabbit anti-Platelet-derived growth factor subunit B (PDGFB) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_002599 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 32, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein PDGFbeta. The membrane blot was probed with anti- PDGFbeta primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein PDGFbeta from E.coli cell (12kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Platelet-derived growth factor beta (PDGFbeta) is a member of Platelet-derived growth factor family which plays an important role in regulating cell proliferation, cellular differentiation, cell growth, development and many diseases including cancer.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat PDGFbeta has not been tested yet.
Related Products
RayBio® Human PDGF-BB ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine (Cat: ELH-PDGFBB-001)
References
- Michael A Beazely, et al. Abelson tyrosine kinase links PDGFbeta receptor activation to cytoskeletal regulation of NMDA receptors in CA1 hippocampal neurons. Mol Brain. 2008; 1: 20.
- Jennifer B. Ptacek, et al. Packing contacts can mediate highly specific interactions between artificial transmembrane proteins and the PDGFbeta receptor. Proc Natl Acad Sci U S A. 2007 July 17; 104(29): 1194511950.
Expiration:
12 months from the date of shipment when stored properly.