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Anti-PLCG2 Antibody
Catalog #: 144-02182
PLCG2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16885 |
| Gene Id | 5336 |
| Gene Symbols | PLCG2 |
| Protein Name / Synonyms | PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2, phospholipase C gamma 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
Format
Calculated MW
147kDa
Observed MW
175kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PLCG2 (NP_002652.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PLCG2 antibody (144-02182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse heart using PLCG2 antibody (144-02182) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLCG2 antibody (144-02182).
Expiration:
12 months from the date of shipment when stored properly.