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Anti-PLK4 Antibody
Catalog #: 144-09863
PLK4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00444 |
| Gene Id | 10733 |
| Gene Symbols | PLK4 |
| Protein Name / Synonyms | PLK4, MCCRP2, SAK, STK18, polo like kinase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
104kDa/105kDa/108kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PLK4 antibody (144-09863) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (144-09863) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.