Anti-PML Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P29590
Gene Id	5371
Gene Symbols	PML
Protein Name / Synonyms	PML, MYL, PP8675, RNF71, TRIM19, protein PML
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	4.93 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

47-48kDa/62-97kDa

Observed MW

98kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PML antibody (144-01184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] PML Polyclonal Antibody (144-01184)

Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (144-01184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] PML Polyclonal Antibody (144-01184)

Immunohistochemistry of paraffin-embedded human gastric cancer using PML antibody (144-01184) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (144-01184)

Immunohistochemistry of paraffin-embedded human uterine cancer using PML antibody (144-01184) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (144-01184)

Immunofluorescence analysis of HeLa cells using PML antibody (144-01184). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] PML Polyclonal Antibody (144-01184)

Expiration:

12 months from the date of shipment when stored properly.

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