Anti-PNCK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6P2M8
Gene Id	139728
Gene Symbols	PNCK
Protein Name / Synonyms	PNCK (C-term)
Clonality	Polyclonal
Preparation	This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human PNCK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Specificity

Other Names

PNCK; Calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase

NCBI Accession #

NP_001034671.3;NP_001129212.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

38500

Recommended Dilutions

IHC: 1:10-50; WB: 1:1000

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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