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Anti-PNKP Antibody
Catalog #: 144-06693
PNKP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96T60 |
| Gene Id | 11284 |
| Gene Symbols | PNKP |
| Protein Name / Synonyms | PNKP, AOA4, EIEE10, MCSZ, PNK, polynucleotide kinase 3'-phosphatase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
53kDa/57kDa
Observed MW
57kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PNKP antibody (144-06693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (144-06693) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (144-06693). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Expiration:
12 months from the date of shipment when stored properly.