Anti-PNLIP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P16233
Gene Id	5406
Gene Symbols	PNLIP
Protein Name / Synonyms	PNLIP, PL, PNLIPD, PTL, pancreatic lipase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	1.45 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

51kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 166-465 of human PNLIP (NP_000927.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PNLIP antibody (144-06396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - PNLIP Polyclonal Antibody (144-06396)

Immunohistochemistry of paraffin-embedded rat kidney using PNLIP antibody (144-06396) at dilution of 1:200 (40x lens).
Immunohistochemistry - PNLIP Polyclonal Antibody (144-06396)

Immunofluorescence analysis of U2OS cells using PNLIP antibody (144-06396). Blue: DAPI for nuclear staining.
Immunofluorescence - PNLIP Polyclonal Antibody (144-06396)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products