PNLIP Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16233 |
| Gene Id | 5406 |
| Gene Symbols | PNLIP |
| Protein Name / Synonyms | PNLIP, PL, PNLIPD, PTL, pancreatic lipase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
51kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 166-465 of human PNLIP (NP_000927.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of BxPC-3 cells, using PNLIP antibody (144-07421) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver cancer using PNLIP antibody (144-07421) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PNLIP antibody (144-07421). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.