Anti-POLB Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P06746
Gene Id	5423
Gene Symbols	POLB
Protein Name / Synonyms	POLB, DNA polymerase beta
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.

Dilutions

WB 1:200 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

38kDa

Observed MW

37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLB antibody (144-01681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] POLB Polyclonal Antibody (144-01681)

Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibody (144-01681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] POLB Polyclonal Antibody (144-01681)

Immunofluorescence analysis of U2OS cells using POLB antibody (144-01681). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] POLB Polyclonal Antibody (144-01681)

Expiration:

12 months from the date of shipment when stored properly.

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