Anti-POLG (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P54098
Gene Id	5428
Gene Symbols	POLG
Protein Name / Synonyms	POLG (C-term)
Clonality	Polyclonal
Preparation	This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

Specificity

Predicted Reactivity

Rat

Other Names

POLG; MDP1; POLG1; POLGA; DNA polymerase subunit gamma-1; Mitochondrial DNA polymerase catalytic subunit; PolG-alpha

NCBI Accession #

NP_001119603.1;NP_002684.1

Other Accession #

Q9QYV8; P54099; NP_001119603.1; NP_002684.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

139562

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50; IHC: 1:10-50

	POLG Antibody (C-term) (Cat# 102-12736) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).
	POLG Antibody (C-term) (Cat# 102-12736) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of POLG Antibody (C-term)(Cat# 102-12736) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	POLG Antibody (C-term) (Cat# 102-12736)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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