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Anti-POLG2 (C-term) Antibody
Catalog #: 102-15175
Rabbit Anti-Human POLG2 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9UHN1 |
| Gene Id | 11232 |
| Gene Symbols | POLG2 |
| Protein Name / Synonyms | POLG2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-376 amino acids from the C-terminal region of human POLG2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).
Specificity
Other Names
POLG2; MTPOLB; DNA polymerase subunit gamma-2, mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta
NCBI Accession #
NP_009146.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
54911
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of POLG2 Antibody (C-term) (Cat# 102-15175) in HL-60 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | POLG2 Antibody (C-term) (Cat# 102-15175) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.