Anti-POLR1C Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O15160
Gene Id	9533
Gene Symbols	POLR1C
Protein Name / Synonyms	POLR1C, AC40, HLD11, RPA39, RPA40, RPA5, RPAC1, RPC40, TCS3, RNA polymerase I subunit C
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

38kDa/39kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR1C (NP_976035.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR1C antibody (144-00269) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - POLR1C Polyclonal Antibody (144-00269)

Immunofluorescence analysis of HeLa cells using POLR1C antibody (144-00269). Blue: DAPI for nuclear staining.
Immunofluorescence - POLR1C Polyclonal Antibody (144-00269)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products