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Anti-POLR1E Antibody
Catalog #: 144-12700
POLR1E Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9GZS1 |
| Gene Id | 64425 |
| Gene Symbols | POLR1E |
| Protein Name / Synonyms | POLR1E, PAF53, PRAF1, RNA polymerase I subunit E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
47kDa/53kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR1E antibody (144-12700) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody (144-12700). Western blot was performed from the immunoprecipitate using POLR1E antibody (144-12700) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.