Anti-POLR2A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P24928
Gene Id	5430
Gene Symbols	POLR2A
Protein Name / Synonyms	POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, RNA polymerase II subunit A
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Dilutions

WB 1:1000 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

63kDa/217kDa

Observed MW

260kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A

Modification

Phospho S2

Storage

-20°C

Images

Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody (144-61509) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - Phospho-POLR2A-S2 pAb (144-61509)

Immunofluorescence analysis of HeLa cells using Phospho-POLR2A-S2 antibody (144-61509) at dilution of 1:100. HeLa cells were treated by nocodazole (50 ng/ml) at 37? for 20 hours (left). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-POLR2A-S2 pAb (144-61509)

Expiration:

12 months from the date of shipment when stored properly.

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