Anti-POLR2C Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P19387
Gene Id	5432
Gene Symbols	POLR2C
Protein Name / Synonyms	POLR2C, RPB3, RPB31, hRPB33, hsRPB3, RNA polymerase II subunit C
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.

Dilutions

WB 1:500 - 1:2000
IHC 1:200 - 1:500
IP 1:20 - 1:50

Format

Calculated MW

31kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR2C antibody (144-01785) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - POLR2C Polyclonal Antibody (144-01785)

Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug POLR2C antibody (144-01785). Western blot was performed from the immunoprecipitate using POLR2C antibody (144-01785) at a dilition of 1:500.
Immunoprecipitation - POLR2C Polyclonal Antibody (144-01785)

Expiration:

12 months from the date of shipment when stored properly.

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