Anti-POLR2D Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O15514
Gene Id	5433
Gene Symbols	POLR2D
Protein Name / Synonyms	POLR2D, HSRBP4, HSRPB4, RBP4, RPB16, RNA polymerase II subunit D
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

16kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR2D antibody (144-01859) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - POLR2D Polyclonal Antibody (144-01859)

Immunofluorescence analysis of A549 cells using POLR2D antibody (144-01859).
Immunofluorescence - POLR2D Polyclonal Antibody (144-01859)

Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody (144-01859). Western blot was performed from the immunoprecipitate using POLR2D antibody (144-01859) at a dilition of 1:1000.
Immunoprecipitation - POLR2D Polyclonal Antibody (144-01859)

Expiration:

12 months from the date of shipment when stored properly.

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