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Anti-POLR2F Antibody
Catalog #: 144-61442
POLR2F Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P61218 |
| Gene Id | 5435 |
| Gene Symbols | POLR2F |
| Protein Name / Synonyms | POLR2F, HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPC15, RNA polymerase II subunit F |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
14kDa
Observed MW
14kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of rat heart, using POLR2F antibody (144-61442) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.