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Anti-POLR2G Antibody

Catalog #: 144-06838

POLR2G Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P62487
Gene Id
5436
Gene Symbols
POLR2G
Protein Name / Synonyms
POLR2G, RPB19, RPB7, hRPB19, hsRPB7, RNA polymerase II subunit G
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
19kDa
Observed MW
20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR2G antibody (144-06838) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - POLR2G Polyclonal Antibody (144-06838)

Expiration:
12 months from the date of shipment when stored properly.