Anti-POLR2H Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P52434
Gene Id	5437
Gene Symbols	POLR2H
Protein Name / Synonyms	POLR2H, RPABC3, RPB17, RPB8, RNA polymerase II subunit H
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:200

Format

Calculated MW

9kDa/12kDa/13kDa/17kDa/19kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR2H antibody (144-06948) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - POLR2H Polyclonal Antibody (144-06948)

Western blot analysis of extracts of various cells, using POLR2H antibody (144-06948) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Western blot - POLR2H Polyclonal Antibody (144-06948)

Expiration:

12 months from the date of shipment when stored properly.

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