POLR2H Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P52434 |
| Gene Id | 5437 |
| Gene Symbols | POLR2H |
| Protein Name / Synonyms | POLR2H, RPABC3, RPB17, RPB8, RNA polymerase II subunit H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
9kDa/12kDa/13kDa/17kDa/19kDa
Observed MW
17kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR2H antibody (144-60291) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using POLR2H antibody (144-60291). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug POLR2H antibody (144-60291). Western blot was performed from the immunoprecipitate using POLR2H antibody (144-60291) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.