Anti-POLR2J Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P52435
Gene Id	5439
Gene Symbols	POLR2J
Protein Name / Synonyms	POLR2J, POLR2J1, RPB11, RPB11A, RPB11m, hRPB14, RNA polymerase II subunit J
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

13kDa

Observed MW

16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR2J antibody (144-01843) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - POLR2J Polyclonal Antibody (144-01843)

Immunofluorescence analysis of U2OS cells using POLR2J antibody (144-01843). Blue: DAPI for nuclear staining.
Immunofluorescence - POLR2J Polyclonal Antibody (144-01843)

Immunofluorescence analysis of A549 cells using POLR2J antibody (144-01843).
Immunofluorescence - POLR2J Polyclonal Antibody (144-01843)

Expiration:

12 months from the date of shipment when stored properly.

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