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Anti-POMC Antibody
Catalog #: RB-08-0013
Rabbit Anti-Human POMC Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_001035256 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human POMC. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells. |
| Reconstitution | Supplied as 200 µg of purified and lyophilized antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 2 ml of water is used, the antibody concentration will be 100 µg/ml. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The proopiomelanocortin (POMC) gene is expressed in the pituitary and arcuateneurons of the hypothalamus. POMC arcuate neurons play a central role in the control of energy homeostasis. The POMC precursor hormone undergoes enzymatic cleavage to yield active ligands for melanocortin receptors. However, processing is incomplete in some tissues,leading to POMC precursor release from cells. POMC gene encodes for several biologically active peptides, including ACTH, beta-lipotropin (beta- LPH), alpha-, beta-, and ?-melanocyte stimulating hormone (alpha-, beta-, ?-MSH) and beta-endorphin, which affect pigmentation; the immune, central, and peripheral nervous systems; adrenocortical function; and energy regulation . Recently, a QTL for leptin serum levels and fat mass was localized to HSA2q21, making POMC a strong positional candidate gene for human obesity studies. Additional studies have shown that mice lacking POMC-derived peptides are obese and have affected adrenal development and pigmentation, much like humans with POMC deficiency. The CNS POMC system has other functions, including regulation of sexual behaviour, lactation, the reproductive cycle and possibly central cardiovascular control.
Specificity
The rabbit anti-human POMC antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
References
- Yaswen, L. et.al. Obesity in the mouse model of pro-opiomelanocortin deficiency responds to peripheral melanocortin.Nature Med. 5: 1066-1070, 1999.
- Santoro, N. et.al. An insertional polymorphism of the proopiomelanocortin gene is associated with fasting insulin levels in childhood obesity. J. Clin. Endocr. Metab. 89: 4846-4849, 2004.
Expiration:
12 months from the date of shipment when stored properly.