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Anti-POP4 Antibody
Catalog #: 144-04452
POP4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95707 |
| Gene Id | 10775 |
| Gene Symbols | POP4 |
| Protein Name / Synonyms | POP4, RPP29, ribonuclease P protein subunit p29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
25kDa
Observed MW
25kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POP4 antibody (144-04452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using POP4 antibody (144-04452) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.