Anti-POR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P16435
Gene Id	5447
Gene Symbols	POR
Protein Name / Synonyms	POR, CPR, CYPOR, P450R, cytochrome p450 oxidoreductase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

76kDa

Observed MW

77kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POR antibody (144-08142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] POR Polyclonal Antibody (144-08142)

Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (144-08142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] POR Polyclonal Antibody (144-08142)

Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (144-08142) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] POR Polyclonal Antibody (144-08142)

Expiration:

12 months from the date of shipment when stored properly.

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