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Anti-POT1 Antibody
Catalog #: 144-01491
POT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9NUX5 |
| Gene Id | 25913 |
| Gene Symbols | POT1 |
| Protein Name / Synonyms | POT1, CMM10, GLM9, HPOT1, protection of telomeres 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
60kDa/71kDa
Observed MW
75kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 497-634 of human POT1 (NP_056265.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POT1 antibody (144-01491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using POT1 antibody (144-01491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using POT1 antibody (144-01491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using POT1 antibody (144-01491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using POT1 antibody (144-01491) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.