RayBiotech

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ISO 13485:2016 cGMP

Anti-POU4F3 Antibody

Catalog #: 144-07712

POU4F3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q15319
Gene Id
5459
Gene Symbols
POU4F3
Protein Name / Synonyms
POU4F3, BRN3C, DFNA15, DFNA42, DFNA52, POU class 4 homeobox 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
37kDa
Observed MW
37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human POU4F3 (NP_002691.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of Jurkat cells, using POU4F3 antibody (144-07712) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - POU4F3 antibody (144-07712)

Expiration:
12 months from the date of shipment when stored properly.