RayBiotech

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ISO 13485:2016 cGMP

Anti-PPIA Antibody

Catalog #: 144-00993

PPIA Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P62937
Gene Id
5478
Gene Symbols
PPIA
Protein Name / Synonyms
PPIA, CYPA, CYPH, HEL-S-69p, peptidylprolyl isomerase A
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
11kDa/18kDa
Observed MW
17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human PPIA (NP_066953.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PPIA antibody (144-00993) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PPIA Polyclonal Antibody (144-00993)

Expiration:
12 months from the date of shipment when stored properly.