3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-PPID Antibody
Catalog #: 144-06949
PPID Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q08752 |
| Gene Id | 5481 |
| Gene Symbols | PPID |
| Protein Name / Synonyms | PPID, CYP-40, CYPD, peptidylprolyl isomerase D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
40kDa
Observed MW
41kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPID (NP_005029.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPID antibody (144-06949) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using PPID Antibody (144-06949) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PPID Antibody (144-06949) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PPID Antibody (144-06949) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using PPID antibody (144-06949). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.