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Anti-PPM1F (C-term) Antibody
Catalog #: 102-17884
Rabbit Anti-Human PPM1F (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P49593 |
| Gene Id | 9647 |
| Gene Symbols | PPM1F |
| Protein Name / Synonyms | PPM1F (C-term) |
| Clonality | Polyclonal |
| Preparation | This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human PPM1F. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.
Specificity
Other Names
PPM1F; KIAA0015; POPX2; Protein phosphatase 1F; Ca(2+)/calmodulin-dependent protein kinase phosphatase; Partner of PIX 2; Protein fem-2 homolog
NCBI Accession #
NP_055449.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
49831
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of PPM1F (arrow) using PPM1F Antibody (C-term) (Cat# 102-17884). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.