Anti-PPP1CC Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P36873
Gene Id	5501
Gene Symbols	PPP1CC
Protein Name / Synonyms	PPP1CC, PP-1G, PP1C, PPP1G, protein phosphatase 1 catalytic subunit gamma
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:200 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

36kDa/38kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PPP1CC antibody (144-04035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)

Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (144-04035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)

Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)

Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)

Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products